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nikon eclipse te2000 s microscope  (Nikon)


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    Structured Review

    Nikon nikon eclipse te2000 s microscope
    Nikon Eclipse Te2000 S Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59970 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nikon+eclipse+te2000/Objectives/bio_rxiv__64898__2026__03__20__713251-226-7-7
    Average 99 stars, based on 59970 article reviews
    nikon eclipse te2000 s microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: A CRISPR/Cas9-Based System for Reprogramming Cell Lineage Specification
    Article Snippet: Alexa Fluor 488 Goat Anti-mouse IgG, Alexa Fluor 568 Goat Anti-rabbit IgG and Alexa Fluor 568 Goat Anti-mouse IgM (Life technologies) were used as the secondary antibody for ICC. .. Fluorescent images were captured using Nikon Eclipse TE2000-U with a Roper Scientific CoolSnap HQ camera or Zeiss 510 inverted confocal microscope. .. Each sample was mixed with 30 μL of Bright-Glo reagent (Bright-Glo Luciferase Assay System, Promega).

    Article Title: Restoration of NOX4 signalling reverses endothelial colony-forming cell angiogenic dysfunction associated with experimental and clinical diabetes
    Article Snippet: Following 24 h incubation at 37 °C, tube networks were imaged with a light microscope for quantification of tube length and branch number using ImageJ based on the average of 3 images (Leica DM2000 LED, 20X magnification) equating to n = 1. .. For 3D tubulogenesis assay, 6 × 10 4 CB-ECFCs were resuspended in growth-factor reduced Matrigel (Corning) mixed 60:40 with complete 12% EGM2 and 40 μl blobs spotted onto 24-well plates, before being left at room temperature for 10 min. Plates were incubated for a further 20 min at 37 °C to polymerise before addition of 1 ml complete media and incubation for 48 h. Blobs were then stained with calcein (2 μg/ml in Hank’s Balanced Salt Solution, HBSS; Thermo Fisher Scientific) for 1 h at 37 °C before transfer to fresh HBSS and imaging using a Nikon Eclipse TE2000-U inverted confocal microscope. ..

    Inverted Microscopy:

    Article Title: Photoswitching endogenous glutamate receptors in neural ensembles and single synapses in vivo.
    Article Snippet: Xenopus embryos were mounted in an oxygenated chamber created with Permanox slides (Sigma-Aldrich) and Gene Frame (ThermoFisher) and bathed in 1X MBS with 0.1 mg/ml MS222. .. Detection of the electroporated OB cells was performed Jo urn l P re- pro of 28 with a Plan Fluor 20X (NA 0.5) objective in a Nikon Eclipse TE2000-U inverted microscope equipped with an EM-CCD camera (Hamamatsu Image Flash 4.0V2 C11440). .. Real-time calcium imaging was performed using a 60X UPLSAPO objective (NA 1.3) in a PerkinElmer Spinning Disk UltraVIEW ERS, Olympus IX81 inverted spinning disk confocal microscope.

    Article Title: Dopaminergic tone inhibits spontaneous glutamate release and augments homeostatic synaptic plasticity
    Article Snippet: .. Fluorescent signals were recorded using a Nikon Eclipse TE2000-U inverted microscope equipped with a 60X Plan Fluor objective (Nikon, Minato, Tokyo, Japan), a Lambda-DG4 illumination system (Sutter Instruments, Novato, CA, USA) with FITC excitation and emission filters, and an Andor iXon+ back-illuminated EMCCD camera (Model no. DU-897E-CSO-#BV; Andor Technology, Belfast, UK). ..

    Article Title: Direct Binding of FGFR3 Autoantibodies to Sensory Neurons Drives Hyperexcitability and Mechanical Pain Hypersensitivity
    Article Snippet: .. Fluorescence imaging was performed with an inverted microscope, Nikon Eclipse TE2000 (Nikon Instruments Inc.), using objective Nikon Super Fluor MTB FLUOR 4× 0.50 and a Nikon Camera (Nikon Instruments Inc.) controlled by NIS Elements software (version 4.20, Nikon instruments). .. The excitation light was delivered by a Lambda-421 system (Sutter Instruments).

    Incubation:

    Article Title: Restoration of NOX4 signalling reverses endothelial colony-forming cell angiogenic dysfunction associated with experimental and clinical diabetes
    Article Snippet: Following 24 h incubation at 37 °C, tube networks were imaged with a light microscope for quantification of tube length and branch number using ImageJ based on the average of 3 images (Leica DM2000 LED, 20X magnification) equating to n = 1. .. For 3D tubulogenesis assay, 6 × 10 4 CB-ECFCs were resuspended in growth-factor reduced Matrigel (Corning) mixed 60:40 with complete 12% EGM2 and 40 μl blobs spotted onto 24-well plates, before being left at room temperature for 10 min. Plates were incubated for a further 20 min at 37 °C to polymerise before addition of 1 ml complete media and incubation for 48 h. Blobs were then stained with calcein (2 μg/ml in Hank’s Balanced Salt Solution, HBSS; Thermo Fisher Scientific) for 1 h at 37 °C before transfer to fresh HBSS and imaging using a Nikon Eclipse TE2000-U inverted confocal microscope. ..

    Staining:

    Article Title: Restoration of NOX4 signalling reverses endothelial colony-forming cell angiogenic dysfunction associated with experimental and clinical diabetes
    Article Snippet: Following 24 h incubation at 37 °C, tube networks were imaged with a light microscope for quantification of tube length and branch number using ImageJ based on the average of 3 images (Leica DM2000 LED, 20X magnification) equating to n = 1. .. For 3D tubulogenesis assay, 6 × 10 4 CB-ECFCs were resuspended in growth-factor reduced Matrigel (Corning) mixed 60:40 with complete 12% EGM2 and 40 μl blobs spotted onto 24-well plates, before being left at room temperature for 10 min. Plates were incubated for a further 20 min at 37 °C to polymerise before addition of 1 ml complete media and incubation for 48 h. Blobs were then stained with calcein (2 μg/ml in Hank’s Balanced Salt Solution, HBSS; Thermo Fisher Scientific) for 1 h at 37 °C before transfer to fresh HBSS and imaging using a Nikon Eclipse TE2000-U inverted confocal microscope. ..

    Imaging:

    Article Title: Restoration of NOX4 signalling reverses endothelial colony-forming cell angiogenic dysfunction associated with experimental and clinical diabetes
    Article Snippet: Following 24 h incubation at 37 °C, tube networks were imaged with a light microscope for quantification of tube length and branch number using ImageJ based on the average of 3 images (Leica DM2000 LED, 20X magnification) equating to n = 1. .. For 3D tubulogenesis assay, 6 × 10 4 CB-ECFCs were resuspended in growth-factor reduced Matrigel (Corning) mixed 60:40 with complete 12% EGM2 and 40 μl blobs spotted onto 24-well plates, before being left at room temperature for 10 min. Plates were incubated for a further 20 min at 37 °C to polymerise before addition of 1 ml complete media and incubation for 48 h. Blobs were then stained with calcein (2 μg/ml in Hank’s Balanced Salt Solution, HBSS; Thermo Fisher Scientific) for 1 h at 37 °C before transfer to fresh HBSS and imaging using a Nikon Eclipse TE2000-U inverted confocal microscope. ..

    Article Title: Direct Binding of FGFR3 Autoantibodies to Sensory Neurons Drives Hyperexcitability and Mechanical Pain Hypersensitivity
    Article Snippet: .. Fluorescence imaging was performed with an inverted microscope, Nikon Eclipse TE2000 (Nikon Instruments Inc.), using objective Nikon Super Fluor MTB FLUOR 4× 0.50 and a Nikon Camera (Nikon Instruments Inc.) controlled by NIS Elements software (version 4.20, Nikon instruments). .. The excitation light was delivered by a Lambda-421 system (Sutter Instruments).

    other:

    Article Title: Pathogenic Role of FGFR3 Autoantibodies in Small Fiber Neuropathy.
    Article Snippet: Less than 24 h after dissociation, DRG neurons were loaded at 37◦C with 3 μM Fura-2AM (Cat#F-1221, Life technologies, stock solution prepared at 1 mM in DMSO, 0.02% pluronic acid-127, Cat#P-3000MP, Life technologies) for 30 min ( λex 340, 380 nm/ λem 512 nm) to follow changes in intracellular calcium ([Ca2 + ]) in Tyrode’s solution (at ≈ 310 mOsm) containing 119 mM NaCl, 2.5 mM KCl, 2 mM MgCl2 , 2 mM CaCl2 , 25 mM HEPES, and 30 mM glucose (pH 7.4).

    Article Title: A CRISPR/Cas9-Based System for Reprogramming Cell Lineage Specification
    Article Snippet: Alexa Fluor 488 Goat Anti-mouse IgG, Alexa Fluor 568 Goat Anti-rabbit IgG and Alexa Fluor 568 Goat Anti-mouse IgM (Life technologies) were used as the secondary antibody for ICC.

    Article Title: Methods for obtaining induced smooth muscle cells
    Article Snippet: Slices were subsequently mounted using Prolong Gold Antifade (Thermo-Fisher Scientific, MA, USA).

    Fluorescence:

    Article Title: Direct Binding of FGFR3 Autoantibodies to Sensory Neurons Drives Hyperexcitability and Mechanical Pain Hypersensitivity
    Article Snippet: .. Fluorescence imaging was performed with an inverted microscope, Nikon Eclipse TE2000 (Nikon Instruments Inc.), using objective Nikon Super Fluor MTB FLUOR 4× 0.50 and a Nikon Camera (Nikon Instruments Inc.) controlled by NIS Elements software (version 4.20, Nikon instruments). .. The excitation light was delivered by a Lambda-421 system (Sutter Instruments).

    Software:

    Article Title: Direct Binding of FGFR3 Autoantibodies to Sensory Neurons Drives Hyperexcitability and Mechanical Pain Hypersensitivity
    Article Snippet: .. Fluorescence imaging was performed with an inverted microscope, Nikon Eclipse TE2000 (Nikon Instruments Inc.), using objective Nikon Super Fluor MTB FLUOR 4× 0.50 and a Nikon Camera (Nikon Instruments Inc.) controlled by NIS Elements software (version 4.20, Nikon instruments). .. The excitation light was delivered by a Lambda-421 system (Sutter Instruments).



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